Multiple conditions of the middle ear are leading to conductive hearing loss, e.g. chronic otitis media, cholesteatoma and otosclerosis, which is regularly investigated by differential diagnosis of several procedures. Current diagnostics cover only single aspects of the pathologies: Otoscopy provides a visual impression and tympanometry the compliance of the tympanic membrane, while audiometry evaluates the overall hearing performance. Nevertheless, all of those tools lack a determination of the leading origin and site of the transmission loss. Accordingly, direct otologic diagnostics enabling the localisation and differentiation for targeted treatment and improved patient outcome are needed.